|
|
|
| Registros recuperados: 237 | |
|
|
| Fonseca,A.S.; Campos,V.M.A.; Magalhães,L.A.G.; Paoli,F.. |
| Low-intensity lasers are used for prevention and management of oral mucositis induced by anticancer therapy, but the effectiveness of treatment depends on the genetic characteristics of affected cells. This study evaluated the survival and induction of filamentation of Escherichia coli cells deficient in the nucleotide excision repair pathway, and the action of T4endonuclease V on plasmid DNA exposed to low-intensity red and near-infrared laser light. Cultures of wild-type (strain AB1157) E. coli and strain AB1886 (deficient in uvrA protein) were exposed to red (660 nm) and infrared (808 nm) lasers at various fluences, powers and emission modes to study bacterial survival and filamentation. Also, plasmid DNA was exposed to laser light to study DNA lesions... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA; Enzyme; Escherichia coli; Filamentation; Laser. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001000011 |
| |
|
|
| Knöbl,Terezinha; Gomes,Tânia A. Tardelli; Vieira,Mônica A. Midolli; Bottino,José A.; Ferreira,Antonio J. Piantino. |
| The occurrence of fim, pap and sfa operons in Escherichia coli isolated from breeders with salpingitis and chicks with omphalitis was evaluated. Analysis of 100 E. coli isolates, by colony hybridization tests, showed that 78 (78%) were fim+, one (1%) was sfa+, seven (7%) were fim+ associated with pap+, eigth (8%) were fim+ and sfa+, one (1%) was fim+pap+sfa+ and five (5%) isolates did not hybridize with any probe. These results suggest that fim adhesion-encoding operon plays an important role in pathogenesis of E. coli infection in chickens with salpingitis and omphalitis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Adhesion-encoding operon; Salpingitis; Omphalitis; Poultry. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200008 |
| |
|
| |
|
| |
|
|
| Casagrande,Leandro; Detanico,Camila Menegon Teixeira; Franco,Robson Maia. |
| A análise de Escherichia coli, considerada bactéria indicadora de contaminação fecal, é utilizada na verificação do controle de processos de abate. Porém, poucos trabalhos foram publicados acerca da ocorrência desse micro-organismo nas indústrias de abate brasileiras. Assim, o presente estudo determinou a ocorrência de E. coli genérica em carcaças bovinas em um estabelecimento sob inspeção federal, habilitado à exportação, identificando as possíveis variações no ano de 2010. Foram coletadas 1111 amostras de suabe de superfície de meias carcaças bovinas e analisadas pela metodologia Petrifilm™. A ocorrência encontrada foi de 4,4% (IC95%=3,3%; 5,7%), com uma média de contagem de 4,08UFC cm-2, não sendo significativamente afetada pelas estações de seca e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abate; Análise bacteriológica; Bovino; Contaminação fecal; Escherichia coli. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000600013 |
| |
|
|
| Wang,Zhongshan; Xiang,Quanju; Wang,Guangjun; Wang,Haiyan; Zhang,Yizheng. |
| The cloning, expression and purification of the glutathione (sulfur) import system ATP-binding protein (gsiA) was carried out. The coding sequence of Escherichia coli gsiA, which encodes the ATP-binding protein of a glutathione importer, was amplified by PCR, and then inserted into a prokaryotic expression vector pWaldo-GFPe harboring green fluorescent protein (GFP) reporter gene. The resulting recombinant plasmid pWaldo-GFP-GsiA was transformed into various E. coli strains, and expression conditions were optimized. The effect of five E. coli expression strains on the production of the recombinant gsiA protein was evaluated. E. coli BL21 (DE3) was found to be the most productive strain for GsiA-GFP fusion-protein expression, most of which was insoluble... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Glutathione transporter; GsiA; Gene expression; Green fluorescent protein. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400019 |
| |
|
|
| Garry, Pascal; Dupont, Jacques; Kaelin, Gaelle; Kergaravat, Cedric; Menanteau, Chantal; Vallade, Emilie; Veron, Antoine; Catherine, Martial. |
| En microbiologie des aliments, le document LAB REF 02 du cofrac demande aux laboratoires de participer au moins deux fois par an aux comparaisons interlaboratoires organisés dans leur domaine analytique afin de démontrer leur compétence et garantir ainsi la qualité de leurs résultats. Par ailleurs, le règlement CE n°882/2004 du 29 avril 2004 relatif aux contrôles officiels précise dans son article 33 que les laboratoires nationaux de référence organisent « des essais comparatifs entre les différents laboratoires nationaux officiels et assurent un suivi adéquat de ces essais ». Dans ce cadre le Laboratoire National de Référence (LNR) microbiologie des coquillages a mis en place des essais d’intercomparaison destinés à l’ensemble des laboratoires réalisant... |
| Tipo: Text |
Palavras-chave: Microbiologie; Analyses microbiologiques; Coquillages; Essais interlaboratoires; Escherichia coli. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00143/25473/23627.pdf |
| |
|
|
| Assis,ACB; Santos,BM. |
| O objetivo desse trabalho consistiu em determinar a patogenicidade in vivo e in vitro de 25 amostras de Escherichia coli obtidas de aves com Síndrome da Cabeça Inchada (SCI). A patogenicidade in vivo foi determinada pela inoculação em pintos SPF de um dia de idade, utilizando-se quatro vias de inoculação: intracardíaca, saco aéreo, oral e traqueal. As aves foram avaliadas quanto à mortalidade a cada 12 horas, por um período de sete dias. Os resultados desse estudo demonstraram que as 25 amostras de E. coli, isoladas de aves com SCI, causaram mortalidade mais elevada em pintos de um dia de idade quando foram inoculadas pelas vias intracardíaca (73,6%) e saco aéreo (68%). As menores taxas de mortalidade em pintos de um dia de idade foram obtidas pelas vias... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Colibacilose; Pintos; Síndrome da cabeça inchada; Verotoxina. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2001000200007 |
| |
|
|
| Cuba,Gabriel Trova; Pignatari,Antonio Carlos Campos; Patekoski,Katya Silva; Luchesi,Lucimila Jorge; Kiffer,Carlos Roberto Veiga. |
| Since antimicrobial resistance among uropathogens against current first line agents has affected the management of severe urinary tract infection, we determined the likelihood that antibiotic regimens achieve bactericidal pharmacodynamic exposures using Monte Carlo simulation for five antimicrobials (ciprofloxacin, ceftriaxone, piperacillin/tazobactam, ertapenem, and meropenem) commonly prescribed as initial empirical treatment of inpatients with severe community acquired urinary tract infections. Minimum inhibitory concentration determination by Etest was performed for 205 Brazilian community urinary tract infection Escherichia coli strains from 2008 to 2012 and 74 E. coli bloodstream strains recovered from a surveillance study. Pharmacodynamic exposure... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pharmacodynamics; Monte Carlo method; Escherichia coli; Urinary tract infection. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000500512 |
| |
|
| |
|
|
| Murer,L.; Ribeiro,M.B.; Machado,L.; Sagave,L.; Kommers,G.D.; Galiza,G.J.N.; Moreira,N.; Lovato,M.. |
| A piometra é uma infecção aguda ou crônica do útero que ocorre frequentemente em cadelas não castradas, podendo também ocorrer em gatas domésticas e selvagens, sendo poucos os estudos relacionados à piometra em grandes felídeos. O objetivo deste relato foi descrever um caso de piometra em uma leoa (Panthera leo) de cativeiro, as lesões de necropsia e histológicas, bem como os resultados da análise microbiológica. Uma leoa com aproximadamente 23 anos, pertencente a um criadouro conservacionista de Santa Maria-RS, foi encontrada morta pela manhã em seu recinto. Após coleta de dados, procedeu-se à necropsia e à coleta de material para análise histopatológica e bacteriológica. A análise microbiológica revelou predomínio das bactérias Streptococcus sp. e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infecção uterina; Grandes felídeos; Streptococcus sp.; Escherichia coli; Septicemia. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300727 |
| |
|
|
| Hernandez-Flores,Jose Luis; Pérez,Juan Caballero; Gutiérrez,Carlos Saldaña; Hernández,Andrés Cruz; Alonso,Gerardo Soto; Hernández,Sergio Pacheco; Gómez,Sergio Romero; Fernández,Francisco; Loske,Achim M.; Guillén,Juan Campos. |
| Abstract Multidrug-resistant microorganisms are of great concern to public health. Genetic mobile elements, such as plasmids, are among the most relevant mechanisms by which bacteria achieve this resistance. We obtained an Escherichia coli strain CM6, isolated from cattle presenting severe diarrheic symptoms in the State of Querétaro, Mexico. It was found to contain a 70 kb plasmid (pMEX01) with a high similarity to the pHK01-like plasmids that were previously identified and described in Hong Kong. Analysis of the pMEX01 sequence revealed the presence of a blaCTX-M-14 gene, which is responsible for conferring resistance to multiple β-lactam antibiotics. Several genes putatively involved in the conjugative transfer were also identified on the plasmid. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Plasmid; Antibiotic resistance; BlaCTX-M-14 gene. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300569 |
| |
|
|
| Allam, L; Ogwu, D; Agbede, R.I.S; Sackey, A.K.B. |
| A study was conducted in 24 gilts to determine the effect of Trypanosoma brucei infection on their reproductive efficiency. The infected gilts developed clinical trypanosomosis following a prepatent period of 2-3 days with 1.8 x 10⁶ trypanosomes per gilt. The clinical signs were observed intermittent fever, pale mucus membranes, short moist cough, moist rales, mucopurulent ocular discharges and hyperemia of the skin, reduced feed intake, and loss of body condition, recumbency, uncoordinated movements, posterior paresis and death of gilts. The cause of death in the pigs was pneumonia caused by Escherichia coli. Grossly, the lungs were severely congested and had undergone gray hepatization. Histopathologically, the lungs had thickened and congested alveolar... |
|
Palavras-chave: Trypanosoma brucei; Immunosuppression; Escherichia coli; Pneumonia; Gilts. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3538 |
| |
|
| |
|
| |
|
|
| Santo,Edilene; Rodolpho,Daniela; Marin,José Moacir. |
| The study was conducted in twenty-three butcheries in the city of Taquaritinga, State of São Paulo, Brazil, surveyed during a 10 months period. Among two hundred and eighty-seven Escherichia coli strains isolated from samples of ground beef, meat-grinding-machines and the hands of manipulators, five were recognized as extraintestinal pathogenic E. coli (ExPEC), showing virulence factors (P and S fimbriae, hemolysin and aerobactin) and presenting multidrug resistance. Retail-sold food may constitute an important vehicle for the dissemination of ExPEC in communities, giving rise to reasons for concern. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; ExPEC; Ground beef; Butchery. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400002 |
| |
|
|
| Fuentes Arriaga,Raquel; Talavera Rojas,Martín; Vázquez Navarrete,Jesús; Soriano Vargas,Edgardo; Gutiérrez Castillo,Adriana. |
| Los alimentos cárnicos constituyen uno de los principales vehículos de enfermedades transmitidas por alimentos, como consecuencia de un manejo deficiente durante su procesamiento. El objetivo del presente trabajo fue determinar la frecuencia de algunos factores de resistencia antibiótica de Escherichia coli en plantas Tipo Inspección Federal (TIF) del Estado de México. Para este fin se analizaron muestras de tres plantas TIF en el Estado de México (n = 90), 10 de materia prima (carne de bovino, cerdo y pavo), 10 de producto terminado y 70 de utensilios de trabajo. Se aislaron 18 (20%) cepas de E. coli, 3 de materia prima, 2 de producto terminado y 13 de utensilios de trabajo (P > 0.05). Las E. coli aisladas presentaron una frecuencia alta de resistencia... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Integrones; Multirresistencia antibiótica. |
| Ano: 2013 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922013000100003 |
| |
|
|
| Bergamini,Alzira Maria Morato; Simões,Marise; Irino,Kinue; Gomes,Tânia Aparecida Tardelli; Guth,Beatriz Ernestina Cabilio. |
| This study aims to assess the prevalence of Shiga toxin-producing Escherichia coli (STEC) in ground beef collected in two cities located in the State of São Paulo, Brazil. A total of 250 samples of raw ground beef were collected in local grocery stores during the period of March to December 2002 in the cities of Ribeirão Preto (114 samples) and Campinas (136 samples), São Paulo State, Brazil. The samples were processed according to standard methods. The resulting 591 E.coli colonies were screened for STEC by hybridization assays using the specific DNA probes, stx1,stx2 and eae. Further characterization of STEC isolates included the search for the ehxA sequence, detection of enterohemolysin and expression of Shiga toxin using the Vero cell assay. STEC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Shiga toxin; STEC; Serotypes; Ground beef. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300032 |
| |
|
| |
|
|
| Rodrigues,J.; Acosta,V.C.; Candeias,J.M.G.; Souza,L.O.; Filho,F.J.C.. |
| In a one-year prospective study carried out to define the role of rotavirus and Escherichia coli in local childhood diarrhea, we determined the prevalence of both agents in 54 diarrheic children attending a health center in Botucatu. Diarrheogenic E. coli (DEC) strains were characterized by O:H serotyping, a search for virulence genetic markers, and assays of adherence to HEp-2 cells. Except for enteroaggregative E. coli (EAEC), no other DEC category was detected in the children's stools. Both EAEC and rotavirus were isolated from 22 of the 54 (41.0%) diarrheic children as single agents or in combination with other enteropathogens. However, when considering the presence of a single agent, EAEC was dominant and isolated from 20.4% of the patients, whereas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Rotavirus; Enteroaggregative E. coli; Escherichia coli; Seasonality. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100008 |
| |
| Registros recuperados: 237 | |
|
|
|
